Preparation and in vitroin vivo evaluation of plga microspheres containing norquetiapine for longacting injection chunwoong park,1 hyojung lee,1 dongwon oh,1 jihyun kang,1 changsoo han,1 dong. Paper title preparation and evaluation of plga microspheres containing bone morphogenetic protein for controlled release zhihong li, jimin wu, pengfei wang, xuezhong chen, shujie huang, jing guan, xizheng zhang institute of medical equipment, academy of military and medical sciences, tianjin 300161, china. Stability studies suggested that the microspheres we prepared had a very good stability. Facile preparation of plga microspheres with diverse internal. Plga is a biocompatible material, and has been used in clinical applications for many years. Preparation, physicochemical characterization, and antitumor activity. Protein encapsulation and preparation of plga microspheres are generally performed using waterinoilinwater w 1 ow 2 double emulsion technique. May 29, 2016 preparation of bsa loaded plga microspheres aq.
The active component can be loaded by means of the physical entrapment, chemical. Pdf to produce poly lacticcoglycolic acid plga microspheres, containing a staphylokinase variant k35r, dgr with reduced immunogenecity and. The loading efficiency, the encapsulation efficiency, and the release profile of the bsaloaded plga microspheres were measured and studied. This study aimed to prepare poly d, llacticcoglycolic acid microspheres plgams by a modified solidinoilinwater sow multiemulsion technique in order to achieve sustained release with. Polyd,llactidecoglycolide plga microspheres were prepared by emulsion solvent evaporation method. Solid biodegradable microspheres incorporating a drug. Research article formulation and evaluation of octreotide acetate loaded plga microspheres l. Preparation and in vitroin vivo evaluation of plga microspheres containing norquetiapine for longacting injection chunwoong park,1. For the primary emulsion, 250 ll of the porogen phosphate buf.
Original article preparation and characterization of lung. Abstractlysozyme, as a model protein, was precipitated through the formation. Preparation of polylactidecoglycolide microspheres and. Hollow microspheres are used as additives to lower the density of a material.
Polydllactidecoglycolide plga has been used to prepare microspheres that possess tremendous potential to release a drug in a controlled manner. The influences of inner aqueous phase, organic solvent, plga concentration on the morphology of microspheres were studied. Studies of plga microspheres pharmaceutical technology. Microsphere can be used for the controlled release of drugs, vaccines, antibiotics, and hormones. Degradex plga particles degradex plga microspheres and nanoparticles are polymeric particles in the size range of nanometers to microns. The entrapment largely depends on the method of preparation and nature of the drug or polymer monomer if used. Pdf preparation and characterization of plga microspheres. Novel preparation method for sustainedrelease plga. Polylacticcoglycolic acid plga particles often serve as a biodegradable and biocompatible platform for drug delivery 1.
Microsphere preparation the microspheres containing the sbm7462 peptide were prepared using the biodegradable plga copolymer 50. The results showed that addition of porogen or surfactants to the inner aqueous phase, types of organic solvents and polymer concentration affected greatly the. The active component can be loaded by means of the physical entrapment, chemical linkage and surface adsorption. Plga, adjusting the parameters of its preparation method, particle. Paper title preparation and evaluation of plga microspheres containing bone morphogenetic protein for controlled release zhihong li, jimin wu, pengfei wang, xuezhong chen, shujie huang, jing guan. Microspheres are manufactured in both solid and hollow. References powders and granulates freeflowing powders and granulates are needed for a variety of industrial processes. Preparation and evaluation of biodegradable microspheres. In the preparation of plga microspheres, using organic solvents, such as chloroform, dichloromethane, acetone, ethyl acetate, glacial acetic acid, and methanol is an unecofriendly technique. Nalmefene was blended with poly lactidecoglycolide.
Polyllactidecoglycolide plga microspheres with diverse internal structures and different release behaviors were prepared via a modified doubleemulsion. Preparation of rifampicinloaded plga microspheres for. Briefly, a sample of freezedried proteinzn complex for l 1 microspheres or freshly prepared suspension for l 2 and l 3 microspheres containing 2 mg of lysozyme was added to 100 mg of plga dissolved in 2 ml anhydrous dichloromethane. The range of techniques for the preparation of microspheres provides multiple options to control as drug administration aspects and to enhance the therapeutic efficacy of a given the drug. This novel method may enhance the bioactivity and ee of proteins because of the decreased interface between oil and water. Preparation of plga microparticles by an emulsionextraction process using glycofurol as polymer solvent. Preparation of compound ornidazolepefloxacin plga microspheres and evaluation of the pharmacological effect on chronic periodontitis rui liu1,2, huili wang3, zhengmou dong1,4, ning liu1,5, xiujie wen1, manjing deng1, faming chen6, luchuan liu1.
Development of composite plga microspheres containing. Pdf microparticles formulated from poly d,llacticcoglycolide plga, a biodegradable polymer, have been investigated extensively as a drug. Common catalysts used in the preparation of this polymer include tinii 2ethylhexanoate, tinii alkoxides, or aluminum isopropoxide. In a previous publication, a mathematical model for microsphere formation using a solvent extraction evaporation method was developed and tested using the experimental data of salmon calcitonin, sct. The influences of inner aqueous phase, organic solvent, plga concentration on. In this process, an active ingredient is first dissolved. Preparation of plga microspheres loaded with leuprolide. Plga microspheres loaded scaffolds were tested for tensile properties in dry condition. Electrospray has proven to be a versatile method to manufacture particles, giving tight control over size.
Microspheres are characteristically free flowing powders having particle size ranging from 1. Pdf preparation of mucoadhesivemicrospheres of atenolol by. Preparation and invitro evaluation of controlled release plga. Microspheres methods for preparation of microspheres. Gastroretentive floating microspheres are lowdensity systems that have. The microspheres constructed from various pla, plga, or plapeg polymers in the presence or absence of bfb0261 were. This solution was emulsified in a vegetable oil, and then amphiphilic agent was added into the emulsion to extract glycofurol and lead to microparticle formation. The terminal group of the plga polymer, where carboxylterminated plga polymers offer faster drug release compared to esterterminated plga. Influence of different formulations and process parameters. It is concluded cpxloaded plga microspheres with suitable characteristics for the treatment of cows with dairy mastitis can be prepared by a wow double emulsion preparation method. Immune augmentation of injectable plgadextran pldex a double polymeric 105 microspheres as an adjuvant for hepatitis b vaccine.
Preparing poly lacticcoglycolic acid plga microspheres. Preparation of microparticles a total of 200 mg of plla and plga three ratios of plla to plga, i. Paper title preparation and evaluation of plga microspheres. Composite plga microspheres containing exenatideencapsulated lecithin nanoparticles exnps plga ms were obtained by initial fabrication of exenatideloaded lecithin nanoparticles exnps via the alcohol injection method, followed by encapsulation of exnps into plga microspheres. Preparation of pllaplga microparticles using solution. Influence of different formulations and process parameters during the preparation of drugloaded plga microspheres evaluated by multivariate data analysis jakub vyslouzil 1, petr dolezel 1, martina kejdusova 1, eliska maskova 1, josef masek 2, robert lukac 2, vratislav kostal 3, david vetchy 1 and katerina dvorackova. During polymerization, successive monomeric units of glycolic. Preparation and characterization of cephalexin loaded plga microspheres volume. Preparation of doxorubicinloaded gasgenerating hollow microspheres plga and tpgs plga hollowmicrospheres were formulatedby using the waterinoilinwater doubleemulsion solvent diffusionevaporation method reported byke and coworkers with slight modifications 19. Preparation of plga microparticles by an emulsionextraction. To obtain a high yield 90% of microspheres with a desirable size and high drug entrapment efficacy, a stirring rate of 8,00010,000 rpm gave the best results. Preparation of plga microspheres with nq freebase nq plga microspheres were prepared by oilinwater ow emulsionsolvent evaporation. Nov 25, 2014 polyd,llactidecoglycolide plga microspheres were prepared by emulsion solvent evaporation method.
We offer degradex plga microspheres and nanoparticles from 100 nm to 50. Use of biodegradable plga microspheres as a slow release. Preparation, characterization, in vitro release and. Plga microspheres and nanoparticles biodegradable polymers. Porous plga microspheres effectively loaded with bsa. The microspheres then dispersed in n hexane, stirred, filtered and dried by lyophilzation 10. Sitosterol loaded plga and pegpla nanoparticles for effective treatment of breast cancer. During some research on plga microspheres we found this interesting article published in european cells and materials vol 7 suppl 2. For example, by taking advantage of the characteristics of microspheres, beyond the basic benefits, the microspheres could provide a larger surface area and.
Drugnanoencapsulated plga microspheres prepared by emulsion. Preparation and in vitroin vivo evaluation of plga microspheres. Influence of different formulations and process parameters during the preparation of drugloaded plga microspheres evaluated by multivariate data analysis jakub vyslouzil 1, petr dolezel 1. First, 1 ml of drug or water was dispersed in 5 ml of different concentrations of plga, pla, and dichloromethane solutions by stirring at a rotation speed of 2500 rpm for one minute using a homogenizer fa25, fluko, shanghai, peoples republic of china. Mar 22, 2012 content introduction advantages polymer used for preparation general method of preparation release of drug from microspheres characterization of microspheres applications 3. Read preparation of rifampicinloaded plga microspheres for lung delivery as aerosol by premix membrane homogenization, international journal of pharmaceutics on deepdyve, the largest.
For example, by taking advantage of the characteristics. Jan 22, 2014 contents of the powerpoint on formulation and evaluation of microspheres include. Drugnanoencapsulated plga microspheres prepared by emulsion electrospray with controlled release behavior shenglian yao 1 school of materials science and engineering, university of science and technology beijing, beijing 83, china. Introduction microspheres are characteristically free flowing powders consisting of proteins or synthetic polymers which are biodegradable in nature and ideally having a. A longacting preparation may address these limitations.
Leuprolide plga microspheres were prepared by two methods, the double emulsionsolvent evaporation method that is employed to manufacture commercially available lupron depot and the more recently developed novel selfhealing microencapsulation method. Content introduction advantages polymer used for preparation general method of preparation release of drug from microspheres characterization of microspheres applications 3. Prediction of solvent removal profile and effect on. The preparation of microparticles consisted in dissolving polymer in glycofurol. Contents of the powerpoint on formulation and evaluation of microspheres include. Gopinath1 1annamacharya college of pharmacy, rajampet, kadapa, 2celon laboratories limited, hyderabad, andhra pradesh, india. Microspheres are made from polymeric, waxy or protective materials that is biodegradable synthetic polymers and modified natural products. In this process, an active ingredient is first dissolved in an aqueous phase w 1 which is then emulsified in an organic solvent of a polymer to make a primary w 1 o emulsion. This research has helped to resolve the technical issues involved in preparation of sustainedrelease microspheres for encapsulating drugs. Drugnanoencapsulated plga microspheres prepared by emulsion electrospray with controlled release behavior shenglian yao. The aim of this paper was to further investigate the in vitro characteristics and in vivo performances of nalmefene microspheres.
Uniformly sized microspheres of polyd, llacticcoglycolic plga encapsulating tamoxifentam were successfully prepared by solvent evaporation. Preparation of plga microspheres with different porous. Blank microparticles were prepared by the wow method. Electrosprays, polylacticcoglycolic acid plga, microspheres we successfully demonstrate the synthesis of polymer microspheres using a single electrospray source, and show their physical characterisation. Microspheres are manufactured in both solid and hollow form.
Injectable and porous plga microspheres that form highly. First, 1 ml of drug or water was dispersed in 5 ml of different concentrations of plga, pla, and dichloromethane solutions by stirring. Drugnanoencapsulated plga microspheres prepared by. Preparation of plga microspheres incorporated gelatin scaffolds aqueous suspension of plga microspheres 0. Preparation and in vitroin vivo evaluation of plga. Preparation and in vitroin vivo evaluation of plga microspheres containing norquetiapine for longacting injection chunwoong park,1 hyojung lee,1 dongwon oh,1 jihyun kang,1 changsoo han,1 dongwook kim21college of pharmacy, chungbuk national university, cheongju, republic of korea. Preparation of microspheres proteinloaded microspheres were prepared by a solvent extraction technique. Design of controlled release plga microspheres for. This novel microencapsulation process overcomes some of the disadvantages associated with the existing methods by. It was shown that the microspheres from double emulsion. Cathelicidinbf30 bf30, a watersoluble peptide isolated from the snake venom of bungarus fasciatus containing 30 amino acid residues, was incorporated in polyd,llactidecoglycolide plga 75. Fabrication of porous microspheres porous plga microspheres were produced by the double emulsion method from 20% wv p dllga 50.
Pdf preparation and characterization of plga microspheres by. We offer a full range of degradex plga microspheres and plga nanoparticles that are ideal for a variety of applications poly d,llactidecoglycolide or plga microspheres and nanoparticles are known to possess a unique ability to enable drug release in a controlled manner. In vitro and in vivo evaluations of plga microspheres. Preparation and characterization of cephalexin loaded plga. Preparation, evaluation and dissolution behaviors of water. Preparation of proteinloaded microspheres using the woo h w method. Preparation and evaluation of mucoadhesivemicrospheres of atenolol and propranolol patil p. Poor patient compliance, untoward reactions and unstable blood drug levels after the bolus administration are impeding the pharmacotherapy for insobriety. Preparation and characterisation of tamplga microspheres. These, however, do not always meet the exacting standards which modern manufacturing. Both plain and fluorescently dyed particles are available. Characterizing release mechanisms of leuprolide acetate.
Leuprolide plga microspheres were prepared by two methods, the double emulsionsolvent evaporation method that is employed to. Electrosprays, polylacticcoglycolic acid plga, microspheres we successfully demonstrate the synthesis of polymer microspheres using a single electrospray source, and show their. Injectable and porous plga microspheres that form highly porous scaffolds at body temperature omar qutachia, jolanda r. The microspheres were obtained by using a solvent diffusion method, the mean diam eter was 9. One gram of plga was dissolved in 5 ml dcm for each batch of microspheres. Original article preparation of compound ornidazole. In the preparation process, the aqueous phase of drugs was. During polymerization, successive monomeric units of glycolic or lactic acid are linked together in plga by ester linkages, thus yielding a linear, aliphatic polyester as a product. In an in vitro study of drug release, it can be concluded that the bdmcplgams exhibited sustained and longterm release properties for 96 h.
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